Britain’s Sonay Kartal missed the chance to reach a home quarter-final when she suffered a tough defeat by American Amanda Anisimova at Queen’s.
World number 50 Kartal was overpowered by the eighth seed in just one hour and five minutes, losing 6-1 6-3.
Kartal had secured the biggest win of her career on Monday against world number 16 Daria Kasatkina.
But a third win over a top-20 player in her career – and a second in the west London tournament – proved a bridge too far.
Anisimova will not have won over much of the British crowd so far at Queen’s with victory over Jodie Burrage in the first round and now Kartal.
She faces compatriot Emma Navarro in the last eight after the third seed beat Beatriz Haddad Maia 1-6 7-6 (7-4) 6-3 earlier on Wednesday.
Earlier, Emma Raducanu and Katie Boulter’s crowd-pleasing doubles partnership came to an end after two matches with defeat in the quarter-finals.
The Britons lost 6-2 7-5 to top seeds Lyudmyla Kichenok and Erin Routliffe in front of a packed court one at Queen’s, which is hosting a women’s event for the first time in 52 years.